cd14-enriched cell fraction  (MBL Life science)

Journal:

Article Title: Lack of Phenotypic and Functional Impairment in Dendritic Cells from Chimpanzees Chronically Infected with Hepatitis C Virus

doi: 10.1128/JVI.78.12.6151-6161.2004

Figure Lengend Snippet: DCs are efficiently propagated in culture medium containing 10% chimpanzee plasma. (A) CD14+ DC progenitors (from HCV-infected chimpanzee 1530) were cultured with IL-4 and GM-CSF in RPMI supplemented with 10% bovine serum or 5 or 10% chimpanzee plasma obtained from an uninfected chimpanzee (A255B or TBDX104). On day 5, a cocktail of cytokines (MCM mimic) was added, and the cells were harvested after 48 h of culture. FACS histograms show profiles for isotype controls (gray areas with solid lines) and monoclonal antibodies (white areas with solid lines). The gates were set to include cells with large amounts of forward scatter (FSC) and side scatter (SSC). The data shown are representative of four experiments. (B) The different groups of DCs (prepared from chimpanzee 1530) were added to 2 × 105 allogeneic T cells in graded doses in triplicate. On day 4, [3H]thymidine was added, and the cultures were incubated for 16 h. The results are expressed in counts per minute. One representative of three experiments is shown.

Article Snippet: PBMCs from leukapheresis samples were used to obtain CD14 + cell-enriched fractions by magnetic bead separation (Miltenyi), and they were cultured for 5 days in medium supplemented with GM-CSF, IL-4, and different doses of chimpanzee plasma (5 or 10% plasma obtained from uninfected chimpanzees) or 10% FCS.

Techniques: Infection, Cell Culture, Incubation